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Custom Antipeptide Antibodies - Europe

These are the antipeptide antibodies available through our custom sythesis service:

Purified Polyclonal Antibodies

Package: 1675€

* It includes :
Peptide synthesis : 10-20 AA, 25 mg, Immunograde
Specific coupling to a carrier : BSA, OVA or KLH
Immunzation of two rabbits : 3 bleedings, ar. 80 ml of serum per rabbit
Affinity purification : Coupling of peptide to column
1 run of 20 ml max (1 rabbit)
Quality control by ELISA and SDS Page

Standard Custom Antipeptide Antibody

Package: 1150€

Peptide + Conjugation + Immunzation
* It includes :
Peptide synthesis : between 10-20 AA, 25 mg, Immunograde
Specific conjugation to a carrier protein : BSA, OVA or KLH
Immunzation of two rabbits : around 80 ml of serum per rabbit
   
You Will Receive :
  • 15 mg of extra peptide delivered with HPLC profile & mass spectrum
  • The remaining conjugate (typically between 1 and 3 mg)
  • 60-70 ml of antiserum per rabbit, the intermediate bleedings included.
To get a personalized quotation click here

To get our tarif, place an order or for any question, contact us at:
ppl@polypeptide.fr - Tel. +33 3 88 79 87 05 - Fax +33 3 88 79 18 56

Immunization Protocol

The following protocol is our standard method of immunization. Please feel free to discuss with us any modifications you would like to propose.

Day 0 14 28 39 56 67 88
Injection 1st 2nd 3rd   4th    
Bleeding Pre-immun     2ml   20ml 50-70ml
For this standard protocol, the average recommended antigen doses per injection and per rabbit is 200 μg.

At the time of the 1st injection we will provide a schedule of immunisation with the exact dates of injections and bleedings.

The scheduled date for the final bleeding will appear on all documents. Complete bleeding is performed with customer's tacit approval 14 days after the second bleeding.

At your request, if antibody titers or serum volume are insufficient, it is possible to continue with one immunisation every 28 days, and to send you the serum 10 days after each new boost.

We can support your custom project from start to finish with gram to multi-kilogram quantities of even the most complex peptides.