Rabbit Polyclonal AntiPeptide Antibodies
PolyPeptide Group will synthesize and purify a peptide sequence of your choice, conjugate the peptide to a carrier protein, immunize three rabbits, test the antiserum, and affinity purify the anti-peptide antibodies.Complete Service includes:
- Synthesis of the peptide up to 20 mer* (customer receives 1-5 mg)
- Conjugation of the peptide to a carrier protein, preferably through a cysteine residue at the N- or C- terminus of the peptide
- Immunization of 3 rabbits and ELISA testing of the serum
- Approximately 225 ml of crude serum
- Affinity purification of 5 ml of crude serum
- Affinity column with peptide attached for subsequent purification
- SDS-PAGE of crude serum and purified antibody
- Complete confidential project report
Polyclonal Antibody Production Protocol:
| Day 0 | Preimmune serum sample and initial immunization |
| Day 14 | 2nd immunization |
| Day 42 | 3rd immunization |
| Day 52 | 1st bleed |
| Day 56 | 4th immunization |
| Day 66 | 2nd bleed |
| Day 70 | 3rd bleed |
Prices:
95% pure: $2,85080% pure: $2,600
70% pure: $2,275
Delivery:
20-22 weeksPolyPeptide Group guarantee:
Our custom anti-peptide antibody service is guaranteed to provide polyclonal antibodies to the peptide sequence ordered, or the antibody portion is provided at no charge.Confidentiality:
All custom work is completely confidential, and the resulting peptide, conjugate and antiserum remain the sole property of the purchaser.*Please inquire for longer sequences
